绿色荧光蛋白作为骨髓间充质干细胞示踪标记物在脑出血脑内的表达

Expression of GFP as a marker of BMSCs at brain of cerebral hemorrhage rats

目的探讨利用绿色荧光蛋白(GFP)作为骨髓间充质干细胞(BMSCs)移植治疗脑出血示踪标记物的可行性。方法利用GFP空载体重组腺病毒感染BMSCs,应用脑立体定位仪将感染的BMSCs移植入脑出血大鼠纹状体内,移植后5、14 d,脑组织行冰冻切片,荧光显微镜下观察BMSCs在脑组织内分布的表达。结果感染细胞与未感染细胞体外培养形态无差异,感染细胞及其传代细胞发强绿色荧光。荧光显微镜下观察脑出血模型冰冻切片,移植后各时间点在移植部位周围病灶区内有大量发绿色荧光的细胞。结论 GFP感染BMSCs能在脑出血大鼠脑内稳定持久表达,表明GFP是脑出血干细胞移植治疗的有效示踪标记物,为进一步研究移植细胞在宿主脑内的可塑性研究奠定基础。

Objective To explore the feasibility of GFP as a marker to trace transplanted BMSCs in the treatment of cerebral hemorrhage.Methods BMSCs were infected by GFP-adenovirus carrier.And infected BMSCs were transplanted into striatum of cerebral hemorrhage rats by stereotaxis instrument,brain tissues were made into frozen sections after transplantation 5,14 d,then the expression of GFP in the brain were observed by fluorescent microscope.Results The morphological feature of the infected BMSCs was similar to that of uninfected BMSCs.GFP was expressed strongly in infected cells and passaged cells.Transplanted cells in cerebral hemorrhage rats at every time piont launched green fluorescent,and they located lesion region around transplantation site.Conclusion GFP can be expressed stablely and persistently at the brain of cerebral hemorrhage rat,so GFP is an effective marker for cell transplantation in the treatment of cerebral hemorrhage,and to establish the foundation for further study the plasticity of transplanted cells at the host brain.