IL-6和PIAS3与肝硬化大鼠肝部分切除后肝再生的关系

Relationship of IL-6 and PIAS3 with liver regeneration in cirrhotic rats after partial hepatectomy

目的研究IL-6和PIAS3在肝硬化大鼠肝部分切除后的变化规律与肝脏再生的关系。方法实验分为肝硬化切除组（E组）和正常肝切除组（C组）,观察比较术后0、1、2、4、12、24、48、72 h肝再生率、增殖细胞核抗原（PCNA）及肝组织IL-6 mR-NA、PIAS3 mRNA的表达。结果术后72 h E组肝再生率明显低于C组（P〈0.05）;E组PCNA的表达在12 h前均高于C组,呈缓慢上升趋势,于48 h达高峰,但远低于C组;E组IL-6 mRNA水平缓慢升高,峰值延迟,且远低于C组峰值;C组PIAS3 mR-NA的水平于术后4 h开始下降,而E组于术后12 h开始下降,且E组术后0~12 h均高于C组（P〈0.05）。结论硬化肝脏部分切除术后肝再生障碍机制与IL-6和PIAS3表达失衡有关。

Objective To explore the change of IL-6 and PIAS3 and their correlation with liver regeneration in cirrhotic rats after partial hepatectomy.Methods SD rats were randomized into 2 groups：group E（cirrhotic rats receiving hepatectomy group）and group C（normal rats receiving hepatectomy group）.We determined liver regeneration rate and expression of proliferating cell nuclear antigen（PCNA）,IL-6 mRNA and PIAS3 mRNA in liver tissue at 0,1,2,4,12,24,48,72 h after operation.Results The liver regeneration rate after partial hepatectomy in group E was significantly lower than that in control at 72 h（P0.05）;the expression of PCNA in group E was higher than that in the group C at 0,1,2,4,12 h,and got to peak at 48 h,but significantly lower than that in the group C at 48 h.The levels of IL-6 mRNA in group E incresed slowly,and the peak value was delayed and significantly lower than that in the group C.The levels of PIAS3 mRNA in group C decreased at 4 h after operation,the group E decreased at postoperative 12 h,and maintained at a higher level than the group C during 12 h.Conclusion The impaired liver regeneration in cirrhotic liver is associated with low expression of IL-6 and high expression of PIAS3.