牛胸腺DNA导入“矮抗58”后代变异及SSR分析

Character Variation of Wheat "AK58" After Introducing Calf Thymus DNA and Assessment by Using SSR Markers

为了创造新的小麦种质资源材料,我们将小牛胸腺DNA通过花粉管通道导入小麦品种"矮抗58",其后代在株高、蜡质层、芒型、穗型、颖壳上有无茸毛、成熟期等几个方面发生大量变异。通过对变异后代系统选择得到多个稳定的变异种质系;通过对DAK58-66、DAK58-34两个种质系的SSR分子标记检测,引物xgwm213、xgwm219、xg-wm400、xgwm428、xgwm148、xgwm408检测出了较丰富的多态性DNA片段,其中xgwm213、xgwm428、xgwm148在DAK58-66、DAK58-34基因组DNA中检测出了小牛胸腺DNA特有而"矮抗58"没有的特异带型,进一步说明了DAK58-66、DAK58-34是由小牛胸腺DNA片段嵌入"矮抗58"基因组DNA变异而来。

To create the new wheat germplasms,we introduced calf thymus DNA into the wheat ＂AK58＂ through the pollen-tube pathway.A large number of variations in plant height,waxiness,awn and ear types,hair on glume and maturation period were found in the descendants.We had owned several unaltered germplasm lines by subsequent continuous selection in the variational descendants;We had detected the DNA of DAK58-66 and DAK58-34 by using SSR markers.A large number of differential fragments had been found by the primers xgwm213,xgwm219,xgwm400,xgwm428,xgwm148 and xgwm408.Especially,we had found the differential fragments that belong to the thymus DNA of calf only in the DNA of DAK58-66 and DAK58-34 by primers xgwm213,xgwm428 and xgwm148.It further showed that DAK58-66 and DAK58-34 came from ＂AK58＂by inlaiding the fragments of thymus DNA of calf.