中国大陆福寿螺种群遗传学研究

Population genetics of Pomacea spp. in mainland of China

目的通过遗传标记分析中国大陆福寿螺种群结构特点,为研究其侵入途径和扩散模式提供基础。方法利用CO I基因扩增引物对中国大陆60个采集点的581个福寿螺标本进行遗传学分析。利用DnaSP 5.10.01进行单倍型多样性和核酸多样性分析,并通过Network 4.2.0.1进行单倍型网络分析。综合GenBank上可利用的福寿螺单倍型和本研究获得的单倍型进行进化关系分析,了解中国大陆福寿螺的进化地位。结果共获得556条有效序列,分属25个单倍型,其中6个单倍型频率较高,占整个样本的96.0%。进化分析表明我国存在Pom cea canaliculata和P.insula-rum2个种。P.insularum单倍型与国外报告类型存在较大差异。结论中国大陆福寿螺种群结构复杂,可能有多种来源和扩散模式。

Objective To reveal the population structure of Pomacea spp.using genetic markers so as to provide the evidence for studying the invasion and expansion of it in the mainland of China.Methods The genetics of 581 specimens of Pomacea spp.from 60 sites was analyzed by sequencing CO I gene.The diversity of nucleotide and haplotypes were calculated in DnaSP 5.10.01.The haplotype network analysis was performed in Network 4.2.0.1.A phylogenetic tree was produced based on the haplotypes from the present study and those available from GenBank in order to understand the taxonomic status of Pomacea spp.in China.Results A total of 556 sequences were acquired in the present study and produced 25 unique haplotypes.Six haplotypes frequently occurred in the specimens and accounted for 96.0%.The phylogenetic analysis identified two Pomacea species in China,i.e.P.canaliculata and P.insularum.The usage of haplotypes of P.insularum in China reversed from the existing pattern in other countries.Conclusion The complexity of population structure of Pomacea spp.in the mainland of China indicates multi-original introduction and complicated expansion patterns.