桑桂通痹剂对胶原诱导关节炎小鼠病理形态学及血管内皮生长因子、基质金属蛋白酶3的影响

Effect of Sanggui Tongbiji on Pathomorphology and Expression of VEGF and MMP-3 in the Joint of CIA Mice

目的观察桑桂通痹剂对胶原诱导关节炎(CIA)模型小鼠踝关节血管翳数目、关节炎指数及血管内皮生长因子(VEGF)、基质金属蛋白酶3(MMP-3)的影响,探讨其作用靶点。方法将50只DBA/1J小鼠随机分为正常组、模型组、桑桂通痹剂组、甲氨蝶呤组、雷公藤多甙片组,除正常组之外,其余各组使用Ⅱ型胶原诱导小鼠造成CIA模型,桑桂通痹剂组、甲氨蝶呤组、雷公藤多甙片于造模后开始给药,共计28 d。按评分法评价关节炎指数,病理观察小鼠踝关节血管翳数目,免疫组化法检测VEGF、MMP-3蛋白表达。结果桑桂通痹剂组可降低模型小鼠的关节炎指数;减少由滑膜细胞和毛细血管增生所形成的血管翳数目,VEGF、MMP-3表达也有所降低。结论桑桂通痹剂可能是通过抑制VEGF、MMP-3表达,减少模型小鼠的关节炎指数和血管翳数目,从而起到治疗作用。

Objective To observe the effect of Sanggui Tongbiji on arthritis index, number of pannus and the expression of VEGF and MMP in the joint of CIA model mice, and explore its action target. Methods Fifty DBA/1J mice were randomiy divided into normal group, model group, Sanggui Tongbiji group, MTX group and T2 group. Expect the normal group, other groups were given type II collagen to made CIA model. The number of pannus and degree of cartilage in the joint were counted, and arthritis index was recorded. The protein expression of VEGF and MMP-3 of all the groups were measured by immunohistomechstry after 28 d treatment. Result The arthritis index was reduced and the retrogression of cartilage was relieved in the substratum of cartilage in the tarsi joint by Sanggui Tongbiji. The expression of VEGF and MMP-3 were also reduced by the treatment. Conclusion Sanggui Tongbiji can down regulate the expression of VEGF and MMP-3, reduce the number of vessel pannus and degree of cartilage in the CIA model, thus play the role of treating CIA.