摘要
目的采用高效液相色谱法建立山楂化滞丸中熊果酸的含量测定方法。方法采用高效液相色谱法,Diamonsil ODS1 C18柱,以乙腈-水-磷酸(85∶14.95∶0.05)为流动相,流速为1.0 mL/min,检测波长为210 nm。结果熊果酸在0.512 6～10.252μg范围内呈良好线性,回归方程为Y=28 451.85X+759.46,r=0.999 8。平均加样回收率为98.9%,RSD=1.13%(n=6)。结论本方法简便、准确,专属性强,测定结果重复性好,为山楂化滞丸中熊果酸的定量分析提供了有效方法。
Objective To establish the method for detemining the content of ursolic acid in Shanzha Huazhi Wan by HPLC. Methods Diamonsfl ODS1 C18 column was used with acetonitrile-water- orthophosphoric （85 ： 14.95 ： 0.05） as the mobile phase, detection wavelength as 210 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.512 6~10.252 μg for ursolic acid and linear equation was Y=28 451.85X＋759.46, r=0.999 8. The average recovery was 98.9% and the related standard deviation （RSD） was 1.13% （n =6）. Conclusion This method was simple, accurate and proper, and the reduplication of the result was good which provide scientific quantitative analysis method of ursolic acid in Shanzha Huazhi Wan.
出处
《中国中医药信息杂志》
CAS
CSCD
2011年第5期49-50,共2页
Chinese Journal of Information on Traditional Chinese Medicine
