桑寄生有效部位对白血病细胞株K562抑制作用的研究

Inhibitory Effect of the Active Sites of Parasitic Loranthus on the K562 Leukemic Cell Line

目的筛选桑寄生对白血病细胞株K562的抑制作用的有效部位。方法采用MTT法和细胞形态学观察的方法,测定桑寄生不同溶剂萃取物体外对K562细胞的抑制作用,确定其有效部位。结果桑寄生正丁醇部位、氯仿部位、乙酸乙酯部位、乙醚部位及桑寄生水提物显示显著的抑制作用,且呈时间-剂量依赖性,其中乙酸乙酯部位400μg/mL浓度作用72h的抑制作用最明显,对白血病细胞株K562的抑制率达80.07%。结论桑寄生的多种溶剂的萃取物在体外对K562细胞有抑制增殖的作用。

Objective To screen inhibitory effect of the active sites of the parasitic loranthus on the leukemic cell K562.Methods Morphological and MTT colorimetric assays were used to explore the inhibitory effect of the Parasitic loranthus on the leukemic cell K562.Results The n-butyl alahol extract,chloroform extract,ethyl acetate extract,aether extract and water extract of the parasitic loranthus of different time-concentration could obviously inhibit the growth of the leukemic cell K562 and the inhibition ratio of the ethyl acetate extract of the parasitic loranthus which effect 72 hours at the concentrations of 400μg/mL was 80.07%.Conclusion The parasitic loranthus could inhibit proliferation of K562 cells in vitro.