免疫荧光双标记监测白血病微小残留病临床研究

The Application of Double Color Immunofluorescence To Monitoring Leukemic Minimal Residual Disease

目的 :观察 Td T及系列相关抗原双标记在急性白血病细胞中的表达及其在预测白血病复发过程中的作用。方法 :采用免疫荧光双标记技术 ,配合流式细胞术检测急性白血病细胞的 Td T及系列相关抗原双标记及急性白血病完全缓解后免疫双标记抗原变化。结果 :64例急性白血病中免疫双标记表达 36例 ( 56.2 5% ) ,其中 32例 AL L全部表达免疫双标记 ,32例 AML 仅有 4例表达( 12 .5% ) ,在 12例获 CR的表达免疫双标记的急性白血病中 ,4例免疫双标记细胞占单个核细胞0 .0 1%～ 0 .0 8% ,并有长期缓解 ,而 8例免疫双标记细胞占单个核细胞 1.5%～ 95.38%的急性白血病 ,均于近期骨髓复发。结论 :免疫荧光双标记技术在部分急性白血病的微小残留监测中具有一定的临床意义。

Objective:To observee the coexpresson of TdT and lineage-associated antigens by acute leukemia and their implication in monitoring the relapse of acute leukemia.Method:The double color of immunofluorescence in cojunction with flow cytometry.Result:In 64 patients with acute leukemia,36 patients coexpressed TdT and lineage-associated antigens accounging for 56.25 %.32 patients with acute lymphoblastic leukemia (ALL) coexpressed TdT and lineage-associated antigens whereas only 4 in 32 patients coexpressed antigens mentioned above accounting for 12.5 % In 12 patients obtaining CR who expressed antigens mentioned above,4 patients expressed 0.01 ~ 0.08 percentage of those antigens and experienced continue CR.Othetr 8 patients expressed 1.5 ~ 95.38 percentage of those antigens showed bone marrow relapse after a short period of CR.Conclusion:TdT and lineage-associated antigens are expressed mainly by ALL,and may be applied to monitoring leukemic MRD.