乙肝血清标志物模式与HBV—DNA含量的关系探讨

Correlation between HBV serological markers and HBV-DNA

目的探讨不同的乙肝血清标志物（HBV—M）模式与HBV—DNA定量检测结果之间的关系。方法选择800例经酶联免疫吸附试验（ELISA）检测HBsAg结果为阳性的血清样本，采用实时荧光定量PCR技术（FQ—PCR）检测其HBV—DNA含量；并根据患者HBV—M的不同模式进行分组统计，分析探讨HBV—M模式与HBV—DNA含量之间的关系。结果不同HBV—M模式中HBV—DNA阳性检出率和含量存在明显差异。①“大三阳”组HBV—DNA含量以中、高拷贝为主；②“小三阳”组及HBsAg（＋）＆Antl—HBc（＋）组HBV—DNA含量以中、低拷贝为主；③单纯HBsAg（＋）组HBV-DNA的检出率仅为2，98％。④HBeAg（＋）血样中HBV—DNA的阳性检出率高达98．33％（118／120），两者之间存在明显正相关（r=0．993）。结论HBV—M与HBV—DNA的检测都能反映HBV感染、传染性及体内复制的情况；HBeAg与HBV—DNA含量存在明显正相关．两者联合检测对乙肝的临床诊疗具有重要指导意义。

Objetive To analyze the correlation between the level of HBV serological markers and HBV-DNA. Methods Serum samples of 800 patients from Xinyi Pepole＇s Hospital clinic department suffering HBV were detected by ELISA and HBV-DNA were detected by FQ-PCR, then analyze the correlation between HBV serological markers and HBV-DNA. Results The HBV-DNA was detected in some HBV serological markers groups and each group was different in the result. HBsAg positive, such as HBsAg, HBeAg and Anti-HBc positive samples has higher amount of copies of HBV-DNA; the low level of HBV-DNA copies were detected in the group of HBsAg, HBeAg or Anti-HBe, Anti-HBc positive; and the HBV-DNA copies rate is 2.98% in the single HBsAg positive. Among 120 HBeAg positive samples, there were 118 HBV-DNA positive, the positive rate of HBV-DNA was 98.33% and the level of HBeAg positive was correlated to HBV-DNA（r=0.993）. Conclusion Both the detection of HBeAg and HBV- DNA cab reflect the active level of duplicate of HBV-M, The HBV-DNA level showed an obvious corralstion to the HBeAg contend in sere.