卷柏启动培养研究

Study on Primary Culture of Selaginella tamariscina

以卷柏(Selaginella tamariscina)幼叶、茎尖及茎段为外植体,研究了不同消毒方式的消毒效果以及基本培养基类型和激素组合对初代培养的影响。结果表明:以卷柏的茎尖为外植体,用5 g.L-1多菌灵处理8 h的消毒方式,取得了较好的消毒效果,无菌率可达79.8%;茎尖最佳初代培养基为MS+KT 2 mg.L-1+NAA0.2 mg.L-1+IAA 0.1 mg.L-1。

Young leaf,shoot tip and stem were used as explants to study the effect of disinfection methods and combinations of basic medium and different hormone on the primary culture of Selaginella tamariscina.The results showed that using shoot tip of Selaginella tamariscina as explants,the highest survival rate of the explants was 79.8% after the explants sterilized in 5 g·L-1 carbendazim for 8 hours;The MS medium containing 2 mg·L-1 KT 0.2 mg·L-1 NAA and 0.1 mg·L-1 IAA was most efficient in the primary culture of shoot tip.