仙珍骨宝胶囊对泼尼松致雄性大鼠骨质疏松的预防作用

Skeletal Effect of Xianzhen gubao on Preventting Prednisone induced Osteoporosis in Male Rats

目的:探讨中药复方制剂仙珍骨宝对糖皮质激素致大鼠骨质疏松的影响,并比较量效关系。方法:36只3月龄♂SD大鼠,随机分为激素模型(灌胃醋酸泼尼松4.5mg·kg-1,2次/周)和仙珍骨宝高、中、低剂量组(0.2,0.4,0.8g·kg-1·d-1ig6次/周),实验于第90天处死,取胫骨上段行不脱钙骨制片骨形态计量学分析。结果:激素模型组较年龄对照组骨量减少,破骨细胞增多,骨形成参数减少,仙珍骨宝组与糖皮质激素模型对照,明显抑制骨吸收,且骨形成参数增加,阻止骨量的丢失(%Tb.Ar+91.23%),高剂量有明显疗效,中低剂量无效。结论:仙珍骨宝胶囊有预防糖皮质激素引起大鼠骨丢失的作用,高剂量疗效显著。

Objective:To study the effect of Chinese medicine Xianzhengubao (XZGB)capsules of compound prescription on corticosteroid induced osteoporosis in rats.Method:3 month old SD  rats were randomly divided into basal control and aging control,prednisone (4.5mg·kg 1 ,ig,twice a week)and XZGB capsules were used in three different doses (0.2,0.4,0.8g·kg 1 ·d 1 ig)6 times a week.All the rats were killed.on the 90th day afteradministration. The proximal tibiae were processed in undecalcified scctions at a thickness of 4～10μm for histomorphometric analysis Result:Compared with the aging controls corticosteroid reduced markedly the trabecular bone mass due to increase of bone resorption and decrease of bone formation (%Tb.Ar 52.9%).Treatment with XZGB capsules in high doses increased significantly the trabecular area as compared with the hormone control(% Tb.Ar+91.2%).The protective mechanism of XZGB capsules mainly consists in suppressing bone resorption and mildly increasing bone formation in rats.Conclusion:Xianzhen gubao capsules have an effect on preventing bone loss in rats induced by corticosteroid,and higher doses produce greater effect.