小分子双水相浮选高效液相色谱法测定污水中痕量氯霉素

Separation and determination of chloramphenicol in sewage using a small molecules-salt aqueous two-phase flotation system coupled with HPLC

用乙醇/磷酸氢二钾双水相体系浮选污水中的氯霉素,通过对盐的质量分数、有机溶剂的体积、气体流速和浮选时间的选择和优化,在理想条件下,氯霉素的平均浮选效率可以达到94%。该方法用于河水和鱼塘水样品中氯霉素的检测,检出限为0.5 ng/mL,加标回收率为89.44%～100.16%,相对标准偏差RSD为1.1%～2.1%,适用于污水中痕量氯霉素的分离/富集及分析测定。

C2H5OH/K2HPO4 two-phase aqueous system was used to floate CAP in sewage.By selecting and optimizing mass percentage of salt,volume of organic solvent,gas flow rate and flotation time,under the optimum conditions,the average floatation efficiency was up to 94%.The method used to detect CAP in the samples of river and pond water,the detection limit was 0.5 ng/mL,the relative recovery was 89.44%～100.16% with relative standard deviation RSD=1.1%～2.1%.The method is applicable to analyze the trace CAP in swage.