摘要
本文制备了与透明质酸（ｈｙａｌｕｒｏｎｉｃ ａｃｉｄ ， Ｈ Ａ） 呈高度特异亲和的结合蛋白， 用氯胺 Ｔ 法碘化标记 Ｈ Ａ 衍生物。采用平衡加样程序， 固相第二抗体作分离剂建立了 Ｈ Ａ 放射免疫分析方法， 其最低检测限为８μｇ／ Ｌ， 最大结合率达９１２ ％ ， 且加样步骤少， 检测时间２ｈ ， 有效期达２ 个月。应用此方法检测１３２ 例肝炎患者血清 Ｈ Ａ 水平， 慢性迁延性肝炎（ Ｃ Ｐ Ｈ） 、慢性活动性肝炎（ Ｃ Ａ Ｈ） 及肝硬化（ Ｌ Ｃ） 平均分别为１９７７ 、４３３５ 和６８５２μｇ／ Ｌ。 Ｈ Ａ 对 Ｃ Ｐ Ｈ 与 Ｃ Ａ Ｈ 有鉴别诊断价值。应用 Ｒ Ｏ Ｃ曲线确定 Ｌ Ｃ 的诊断限为４５０μｇ／ Ｌ， 其灵敏度为９５７ ％ 、特异性７８５ ％ 。说明本方法稳定可靠， 对肝纤维化有诊断作用。
Hyaluronic Acid(HA) specific binding protein(HABP) was extracted and purified HA derivative was labeled with 125 I using chloramine T method A new radioimmunoassay for HA was established based on the aggregation of HA with HABP,followed by binding to anti HABP antibody and solid second antibody sequentially within 2 hours The lowest limit of detection for the assay was 8μg/L Serum HA levels of 132 patients with hepatitis showed that it could differentiate chronic active hepatitis from chronic persistent hepatitis ROC curve revealed that HA had a good diagnostic performance for liver cirrhosis with sensitivity of 95 7% and specificity of 78 5% at cutoff value of 450μg/L We conclude that the present method is sensitive,reliable and useful for chronic hepatitis
出处
《上海免疫学杂志》
CSCD
北大核心
1999年第4期216-218,共3页
Shanghai Journal of Immunology
关键词
透明质酸
放射免疫分析
肝纤维化
肝硬化
hyaluronic acid
radioimmunoassay
hepatic fibrosis
liver cirrhosis
