清胆胶囊对胆石病C57小鼠肝脏基因表达谱的影响

Effects of "Qingdan Capsule" on liver gene expression profiles in C57 mice with cholelithiasis

目的探讨清胆胶囊对胆石病C57小鼠肝脏基因表达谱的影响。方法将38只SPF级C57BL/6J雌性小鼠随机分为对照组(n=10)、模型组(n=15)及清胆胶囊组(n=13)。对照组喂以正常饲料,模型组和清胆胶囊组喂以高脂致石饲料,其中清胆胶囊组在予高脂饮食的同时,予清胆胶囊干预。于实验第8周末处死,应用Oligo GEArray芯片检测肝脏基因表达的改变。结果与对照组比较,模型组出现显著差异表达的基因共35条,上调基因34条,下调基因1条,为Col3a1;与模型组比较,清胆胶囊组出现显著差异表达的基因38条,其中下调基因36条,上调基因2条,为Il3和Ppard;与模型组比较,对照组和清胆胶囊组同时下调两倍的基因27条,主要为代谢酶相关基因、脂肪酸结合蛋白基因、炎症和纤维化相关基因等。结论从基因学角度分析显示,清胆胶囊可以改善肝脏的炎症损伤,减少胆汁中胆固醇结晶的形成,降低结石形成的几率。

Objective To observe the effects of ＂Qingdan Capsule＂ on liver gene expression profiles in C57 mice with cholelithiasis.Methods Thirty-eight SPF C57BL/6J female mice were randomly divided into three groups： control group in which 10 mice were fed normal diet,model group in which 15 mice were fed high-fat diet and treatment group in which 13 mice were fed high-fat diet and ＂Qingdan Capsule＂.All mice were sacrificed at the 8th week and hepatic gene expression was detected with Genechip Oligo GEArray〖XCR.tif;%70%70〗.Results Compared with control group,the number of significant different expression genes in model group was 35 including 34 of up-regulated genes and 1 down-regulated gene（Col3a1）;compared with model group the number of significant different expression genes in treatment group was 38 including 36 of down-regulated genes and 2 up-regulated genes（Il3 and Ppard）;compared with model group the number of down-regulated genes in treatment group and control group was 27,such as metabolic enzyme genes,fatty acid binding protein genes,inflammatory genes and fibrosis genes.Conclusion Treatment of cholelithiasis with ＂Qingdan Capsule＂ can relieve inflammation,reduce the cholesterol crystallization in bile and lower risk of gallstone formation.