高效液相色谱法测定白牦牛乳B族维生素的方法研究

Content determination of B vitamins in white yak milk by HPLC

以ODS-C18（5μm,4.6 mm×150 mm）为色谱柱,庚烷磺酸钠离子对试剂（PIC-B7）和甲醇为流动相并采用梯度洗脱,在流速为1 mL.min-1、柱温为30℃条件下,用波长为275 nm的紫外检测器进行高效液相色谱法（HPLC）检测和分析白牦牛乳中B族维生素的含量.结果表明：白牦牛乳中维生素B3的含量最高（345.588 6μg.（100 mL）-1）,维生素B11的含量最低（4.815 7μg.（100 mL）-1）;B族维生素的平均回收率为82.98%~97.96%.在本研究所建立的样品前处理方法和HPLC分析色谱条件下,能快速、准确地同时测定白牦牛乳中各种B族维生素的含量,具有重现性好和回收率高的特点.

The high performance liquid chromatography method for a simultaneous contents determination of B vitamins in white yak milk had been developed.Separation and contents determination of B vitamins were achieved by using ODS-C18 column（5 μm,4.6 mm×150 mm）,gradient elution with sodium heptanesulfonate（PIC-B7 reagent） and methanol as mobile phase at the flow of 1 mL·min-1,column temperature 30 ℃ and 275 nm UV.The results showed that the maximal and minimal contents of B vitamins were B3（345.588 6 μg·（100 mL）-1） and B11（4.815 7 μg·（100 mL）-1） respectively.The average recover rate of B vitamins was 82.98 %～97.96 %.With the ascertained samples pre-dispose method and chromatography conditions in the study,B vitamins in white yak milk have been simultaneous determined quickly and accurately,and with the advantages of better repeatability and higher recovery.