ICR小鼠Mx1基因的克隆及组织表达

Cloning and Tissue Expression of Mx1 Gene in ICR Mouse

为了对ICR小鼠的Mx1基因序列特征进行比较分析,揭示该基因的组织特异性表达规律,用干扰素诱导剂poly（I）/（C）诱导小鼠腹腔巨噬细胞,应用RT-PCR技术克隆小鼠Mx1基因全长编码区序列,并用半定量RT-PCR方法研究该基因在组织中的特异性表达规律。结果显示,从雌性ICR小鼠腹腔巨噬细胞中克隆的Mx1基因的CDS序列大小为1 611 bp,编码536个氨基酸。该序列与其他物种Mx基因序列的比较结果显示,核苷酸同源性为37.36%~69.98%,氨基酸同源性为38.8%~76.6%。半定量RT-PCR显示,Mx1基因在所检测组织的脾脏、肺脏中表达量较高,肝脏中度表达,心脏、肾脏低度表达,肌肉中不表达。研究结果表明,克隆的Mx1基因所编码的蛋白具有脊椎动物Mx蛋白共有的结构特征,该基因的表达具有组织特异性。

This study was carried out to obtain and analyze sequence of ICR mouse Mx1 gene,and to investigate the gene expression patterns of multiple tissues.Full-length sequence of ICR mouse Mx1 gene was amplified by RT-PCR from the total RNA extracted from mouse peritoneal macrophages induced by poly（I） /（C） and its expression in different tissues was studied by semi-quantity RT-PCR.A 1 611 bp cDNA of Mx1 gene encoding 530 amino acids was first cloned from fetal ICR mouse peritoneal macrophages.Analysis of homology showed that the mouse Mx1 gene and the deduced amino acids shared 37.36 % to 69.98% and 38.8% to 76.6% homologies with those from other animals.The semi-quantity RT-PCR revealed ICR mouse Mx1 gene nucleotide sequence displayed high expression in spleen and lung,middle expression in liver,low expression in heart and kidney,no expression in muscle.Mx protein coded by the amplified mouse Mx1 gene had the common characteristics of vertebrates＇ Mx proteins.The expression level of Mx1 gene is particular to tissues.