杠板归药材薄层色谱检测方法研究

Experimental Research on TLC Identification of Polygonum perfoliatum L.

目的:建立杠板归药材的聚酰胺薄层色谱鉴别方法。方法:杠板归经不同提取方法提取后,以甲醇∶甲酸(100∶2)为展开剂,对杠板归中的槲皮素-3-Oβ--D葡-萄糖醛酸进行聚酰胺薄层鉴别。结果:薄层色谱鉴别斑点清晰,能够有效鉴别杠板归中的槲皮素-3-Oβ--D葡-萄糖醛酸。结论:本法操作简便,稳定,专属性强,可有效鉴别杠板归药材,为其质量标准提升打下一定基础。

Objective ： To establish a method of Polyamide Thin Layer Chromatography （TLC） identification for Polygonum perfoliatum L.. Methods ： Polyamide TLC identification and analysis was studied on the various extractions by different solvents,TLC systems was performed for identifying Quercetin-3- O-β-D-glucuronide by： methyl alcohol-methanoic acid （100：2 ）. Results： The TLC spots were fairly clear and it can distinguish Quercetin-3-O-β-D-glucuronide effectively. Conclusion： The method is simple, stable, and specificity and effective for the identification of Polygonum perfoliatum L. , and can be used to control the quality of Polygonum perfoliatum L.