甲硝唑氯己定洗剂中甲硝唑的紫外分光光度法含量测定

Determination of metronidazole in the compound metronidazole-chlorhexidine lotion by UV spectrophotometry

目的:建立甲硝唑氯己定洗剂中甲硝唑含量的紫外分光光度测定法。方法:采用紫外分光光度法,以水为空白对照,检测波长为320 nm,测定甲硝唑的含量。结果:甲硝唑浓度在4.096～12.288μg/m l范围内线性关系良好(r=0.999 9,n=5),平均加样回收率99.16%,RSD为0.88%。结论:该法操作快速简便,结果准确,适用于甲硝唑含量的快速测定及质量控制。

Objective：To establish an ultraviolet spectrophtometry method for the determination of metronidazole in the lotion of chlorhexidine containing metronidazole.Methods：The water was used as solvent.The detection wavelength was set at 320 nm.The contents of metronidazole were determined by ultraviolet spectrophotometry.Results：The calibration curve of metronidazole was linear when its concentration range was from 4.096 to 12.288 μg/ml（r=0.999 9,n=5）,and average recovery was 99.16%,RSD was 0.88%.Conclusion：The method is rapid and simple,the result is precis,and can be used for the quality control and rapid determination of metronidazole lotion.