亚低温对新生鼠缺氧缺血性脑损害不同脑区p16和Bcl-2表达的影响研究

The study of influnce of hypothermia to the expression of P16 and Bcl-2 levels in different brain tissues of the rats with hypoxic-ischemic brain damage.

【目的】探讨亚低温对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)后脑灰质、室周白质的保护作用。【方法】建立新生大鼠HIBD模型,31℃、34℃亚低温全身干预3 h,观察缺氧缺血(hypoxic-ische-mic,HI)后24 h、72 h、7 d脑皮质、海马、室周白质细胞凋亡、p16、bcl-2表达。【结果】1)p16灰度:模型组表达高于假手术组,HI后24 h达高峰,31℃组表达减少,34℃组总表达不减少,但在HI后24 h表达减少,高峰延迟;2)凋亡细胞灰度:与p16结果一致;3)bcl-2灰度:也与p16结果一致,34℃组术后24 h、HI后72 h表达均减少;4)相关关系:p16、凋亡细胞、bcl-2灰度在各脑区表达一致,两两比较,有相关关系(P<0.01)。【结论】新生大鼠HIBD后亚低温干预通过降低p16、bcl-2的表达、减少细胞凋亡或延缓其高峰对脑灰质、室周白质组织起到保护作用。

【Objective】 To investigate the protective effects of hypothermia to the grey matter and the periventricular white matter of the brains of the neonatal rats with hypoxic-ischemic brain damage（HIBD）.【Methods】 The 7d neonatal rats got the HIBD model.Hypothermias of 31 ℃,34 ℃ were given to them for 3 h immediately after the operation.The degrees of grey of apoptosis,p16,bcl-2 of the cerebral cortex,the hippocampi tissue and the periventricular white matter in the brains of rats was used to observe when 24,72,168 h after the operation.【Results】 1）The degrees of grey of p16： It was higher in the HIBD group than that in the pseuoperation group,the peak was at 24 hours after HI,it was deduced in 31 ℃ group,it was not deduced in 34 ℃group,but the peak was delated.2） The degrees of grey of apoptosis： It was the same as that of p16.3） The degrees of grey of bcl-2： It was the same as that of p16,it were deduced 24,72 hours after HI in the 34 ℃ hypothermia group.4） Correlation： there were the correlation ships（P0.01） between either two of the degrees of the greys of p16,apoptosis,bcl-2 in all the brain tissues.【Conclusions】 Hypothermia intervention could obviously deduce the express of p16,bcl-2,deduce apoptosis or delate their peaks of neonatal rats,brains with HIBD,so protected their grey matter tissue and the white matter tissue.