唐古特红景天乙醇提取物对人乳腺癌细胞MCF-7增殖及凋亡的影响

ANTIPROLIFERATIVE AND APOPTOSIS EFFECT ON BREAST CANCER CELL MCF-7 CELLS OF THE ALCOHOL EXTRACT OF RHODIOLA ALGIDA VAR.TANGUTICA

目的研究唐古特红景天75%乙醇提取物对体外培养乳腺癌细胞MCF-7增殖的影响并初步探讨其作用机制。方法采用高效液相色谱法鉴定提取物的有效成分;采用MTT试验检测提取物对MCF-7细胞增殖的影响;采用光镜观察药物处理后细胞形态学的变化;利用Annex-in V-FITC/PI双染色流式细胞术分析药物对细胞凋亡的影响。结果唐古特红景天中有红景天苷和酪醇等成分;在一定的剂量和时间范围内,提取物能抑制MCF-7细胞生长;通过光镜观察,见药物可引起肿瘤细胞出现胞浆空泡和凋亡小体;流式细胞术检测发现,低浓度的提取物可导致肿瘤细胞出现早期凋亡,随着药物浓度增高,晚期凋亡和坏死细胞逐渐增多。结论唐古特红景天75%乙醇提取物可抑制体外培养MCF-7乳腺癌细胞的增殖,其抑制作用与诱导肿瘤细胞凋亡的机制相关。

Objective To study the anti-tumor effect of Rhodiola on breast cancer cell MCF-7 cells proliferation and apoptosis.Methods The effective constituent of the extract was analysed with the high efficiency liquid chromatography.The survival rate of MCF-7 cells was evaluated by MTT test.The morphological change of cells was observed with optical microscope.The apoptotic influence of the extract on cells was researched with Annexin V-FITC/PI kit and flow cytometry.Results The main constituents of the extract contained the salidroside and tyrosol.The extract caused decrease in the survival of MCF-7.The results of optical microscope showed the vacuole and apoptotic body in MCF-7 cells.The FCM analysis showed that lower concentrations of the extract caused much apoptotic cells and higher concentrations led to a lot necrosis of cells.Conclusion The extract of Rhodiola algida Var.tangutica inhibits division of MCF-7 cells and the cytostatic and antiproliferative effect may be related to apoptosis induced mechanism.