NF-κBP65和caspase-3在中耳胆脂瘤中的表达及其临床意义

Expression of NF-κB and caspase-3 in middle ear cholesteatoma and its clinical significance

目的研究NF-κB P65和caspase-3在中耳胆脂瘤中的表达及意义。方法应用免疫组织化学方法检测30例胆脂瘤上皮组织(胆脂瘤组)及10例正常外耳道皮肤组织(对照组)中NF-κB P65和caspase-3蛋白的表达情况,并采用图像分析系统对表达结果进行定量分析。结果免疫组化染色显示胆脂瘤上皮组织中NF-κB P65表达阳性率显著高于正常上皮组织,两组比较差异有统计学意义(P<0.05),其阳性单位分别为27.90±9.13和13.23±9.05,两组间比较差异有统计学意义(t=4.307,P<0.05);胆脂瘤上皮组织中caspase-3表达阳性率显著高于正常上皮组织(P<0.05),其阳性单位分别为17.29±6.21和8.31±3.44,两组间比较差异有统计学意义(t=4.287,P<0.05)。胆脂瘤上皮中NF-κB P65与caspase-3表达无相关性(r=0.01,P=0.961)。结论 NF-κB P65与caspase-3过表达可能与中耳胆脂瘤的发生和发展有关,其可能参与了中耳胆脂瘤上皮细胞的增殖及过度凋亡的调控。

Objective To explore the expression of NF-κB P65 and caspase-3 in middle ear cholesteatoma and its significance.Methods The expression of NF-κB P65 and caspase-3 protein in 30 cases of middle ear cholesteatoma and 10 cases of normal external acoustic meatus skin was detected using immunohistochemical staining method and quantitative analysis of image.Results The positive expression rates of NF-κB P65 protein and caspase-3 were significantly different between two groups（P〈0.05）.The positive units（PU） of expression of NF-κB P65 protein were 27.90±9.13 in middle ear cholesteatoma and 13.23±9.05 in normal external acoustic meatus skin（t=4.307,P〈0.05）.The positive units（PU） of expression of caspase-3 protein were 17.29±6.21 in middle ear cholesteatoma and 8.31±3.44 in normal external acoustic meatus skin（t=4.287,P〈0.05）.The expression of NF-κB P65 was not correlated with expression of caspase-3（r=0.01,P=0.961）.Conclusion The overexpression of NF-κB P65 and caspase-3 may involve in the formation and development of middle ear cholesteatoma by regulating apoptosis and cell proliferation.