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病原芽胞杆菌几丁质酶产生菌的筛选与活性鉴定 被引量：1

Chitinolytic ActivityDetection in Pathogenic Bacillus sp. Strains
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摘要 通过特异引物PCR方法和水解圈活性法对62株苏云金芽胞杆菌菌株、26株蜡状芽胞杆菌菌株及18株球形芽胞杆菌菌株进行了几丁质酶产生菌的筛选。所测苏云金芽胞杆菌中除4株野生型菌株外均为阳性结果,蜡状芽胞杆菌仅1株为阴性结果,球形芽胞杆菌全为阴性结果,且水解圈法观察结果与PCR检测结果一致。在此基础上,通过DNS比色法对几丁质酶产生菌株的几丁质酶比活力也进行了测定。对这些具有致病性的病原芽胞杆菌几丁质酶的研究对于研究其致病机理及对其进行遗传改良具有重要的理论和实际应用价值。 The presence of chitinase gene and the chitinolytic activity among pathogenic Bacillus sp.strains have been detected by PCR method and chitinase activity assays.The results showed that no chitinase gene and chitinase production been observed in 1 of 26 Bacillus cereus strains,4 of 62 B.thuringiensis strains and all 21 B.sphaericus strains.All other strains could produce chitinolytic activity during their growth.The investigation of chitinase-producing strains in pathogenic Bacillus sp.provide us more information for the research of Bacillus sp.strains＇ pathogenesis and chitinase＇ application potency in insect control.
作者 蔡亚君 桂震 李锋 杨小俊
机构地区 武汉纺织大学环境工程学院
出处 《武汉科技学院学报》 2011年第3期58-61,共4页 Journal of Wuhan Institute of Science and Technology
基金 武汉纺织大学校基金项目(2008Z24)
关键词 几丁质酶 苏云金芽胞杆菌 蜡状芽胞杆菌 球形芽胞杆菌 比活力 致病 Chitinase Bacillus thurinngiensis B.cereus B.sphaericus chitinolytic activity pathogenic
分类号 Q89 [生物学]
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参考文献10

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同被引文献30

  • 1岳振峰,蓝芳,谢丽琪.气相色谱-质谱法测定XO酱中BHA、BHT和TBHQ[J].中国粮油学报,2004,19(5):83-85. 被引量：28
  • 2陈三凤,李季伦.几丁质酶研究历史和发展前景[J].微生物学通报,1993,20(3):156-160. 被引量：82
  • 3蒋志强,徐刘平,郭坚华.嗜几丁质类芽孢杆菌菌株CH11几丁质酶特性研究[J].江苏农业科学,2006,34(1):47-49. 被引量：2
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  • 5KEN-ICHI KUROSH1MA, TAKESHI SAKANE, RENKICHI TAWITA, AKIRA YOKOTA. Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov. , New Chitinolytic Members of the Genus Bacillus [J]. International Journal of Systematic Bacteriology, 1996, 46 (1): 76-80.
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  • 7de Ara jo NK, de Assis CF, Dos Santos ES, et al. Production of enzymes by Paenibacillus chitinolyticus and Paenibacillus ehimensis to obtain chitooligosaccharides [J ]. Applied Biochemistry and Biotechnology, 2013, 170 (2) : 292-300.
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武汉科技学院学报
2011年 第3期

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