吸入布地奈德对哮喘大鼠IL-4、IL-10、IFNγ-水平的影响

Effects of budesonide inhalation on IL-4,IL-10,IFN-γ level in asthma rats

目的探讨哮喘大鼠IL-4、IL-10、IFNγ-水平以及布地奈德的干预作用。方法将30只W istar大鼠随机分组(n=10):模型组(A组)采用卵白蛋白致敏与激发;空白对照组(C组),以生理盐水代替OVA;布地奈德组(B组),第六次诱发哮喘结束后30 m in采用布地奈德混悬液雾化吸入2周。模型组和空白对照组以等量生理盐水代替。检测细胞因子IL-4、IL-10、IFNγ-的水平。结果 A组大鼠单核细胞培养上清中IL-4的水平明显高于C组(P<0.05),IL-10、IFNγ-水平明显低于C组(P<0.05);B组大鼠单个核细胞培养上清中IL-4的水平明显低于A组(P<0.05),IL-10、IFNγ-水平明显高于A组(P<0.05)。结论细胞因子IL-4、IL-10、IFNγ-参与哮喘发病的病理生理过程,吸入布地奈德能够有效改善Th1/Th2动态平衡,减轻哮喘的发作。

Aim To explore the effects of budesonide inhalation on IL-4,IL-10,IFN-γ level in asthma rats.Methods 30 Wistar rats wre randomly divided into three groups with 10 rats each group：the asthma group（A group,allergized and stimulated by OVA）,the budesonide group（B group,treated with budesonide inhalation）and the control group（C group,substituted NS for OVA and budesonide inhalation）.The level of IL-4,IL-10,IFN-γ were examined by Elisa Kit.Results Compared with C group,the level of IL-4 in A group rats was significantly high（P0.05）,the levels of IL-10,IFN-γ were significantly low（P0.05）.Compared with A group,the level of IL-4 in B group rats was significantly low（P0.05）,the levels of IL-10,IFN-γ were significantly high（P0.05）.Conclusion IL-4,IL-10,IFN-γ were involved in asthma＇s pathophysiological process.Budesonide inhalation can attenuate asthma seizure through improving dynamic equilibrium between Th1 and Th2.