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Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach

Relationships between two Pseudoalteromonas strains isolated from the Canada Basin and the Southern Ocean using a polyphasic approach
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摘要 The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSwl0014, isolated from the Canada Basin and from the Southern Ocean, respectively, were determined using a polyphasic taxonomic approach. There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared. Nonetheless, they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses. Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level. However, a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas. The evolutionary relationships and taxonomic position of two marine planktonic bacterial strains BSw20211 and BSwl0014, isolated from the Canada Basin and from the Southern Ocean, respectively, were determined using a polyphasic taxonomic approach. There was a close phylogenetic relationship between the two strains and most phenotypic properties were shared. Nonetheless, they were found to belong to different species of the genus Pseudoalteromonas on the basis of genotypic analyses. Findings were consistent with the suggestion that gyrB gene sequence comparison and DNA-DNA relatedness might better define phylogenetic relationships of bacteria at the species level. However, a cut-off value of 90% gyrB gene sequence similarity was not reliable for the differentiation of species within the genus Pseudoalteromonas.
出处 《Chinese Journal of Polar Science》 2011年第1期25-34,共10页 极地研究(英文版)
基金 supported by the National Natural Science Foundation of China (Grant nos. 40676002, 40876097, 41076131) the National High Technology Research and Development Program of China (Grant no. 2008AA09Z408) the Program for Changjiang Scholars and Innovative Research Team in the University (Grant no.40821063) China's Action Plan for the International Polar Year (IPY)
关键词 PSEUDOALTEROMONAS phylogenetic relationship 16S rRNA GYRB DNA relatedness Pseudoalteromonas, phylogenetic relationship, 16S rRNA, gyrB, DNA relatedness
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