正交试验法优化蚯蚓生长和繁殖的工艺研究

Optimization of Growth and Reproduction of Earthworm Using Orthogonal Test

试验以猪粪为饵料,以蚯蚓的总生物量为衡量指标;以牛粪为饵料,以蚯蚓的总产茧量为衡量指标。选择温度、湿度、接种密度、C/N比为单因素水平,通过正交试验L9(34),分别对猪粪条件下蚯蚓的总生物量和牛粪条件下蚯蚓的总产茧量进行了研究。结果表明,以猪粪为饵料时,温度为20℃,接种密度为12条/90g干饵料,湿度为70%,C/N比为14.2,蚯蚓的总生物量最高,极差分析表明,接种密度的影响较大;以牛粪为饵料时,温度为25℃,接种密度为12条/90g干饵料,湿度为65%,C/N比为19.4,蚯蚓的总产茧量最大,极差分析表明,温度的影响最大。

When pig excrement was used as bait,the total biomass of earthworms（Eisenia foetida） was used as an evaluating indicator.When cow dung was used as bait,the total cocoon production of earthworms（Eisenia foetida） was used as an evaluating indicator.The factors included temperature,humidity,inoculum density and ratio of C/N.The orthogonal test L9（34） was used for experimental design.The results showed that the total biomass of earthworms peaked when using the pig excrement as bait under the following conditions：temperature,20 °C;inoculum density,12 earthworms per 90 g dry bait;humidity,70%;and ratio of C/N,14.2.Range analysis showed that the inoculum density had greater effects on the total biomass of earthworms.The total cocoon production of earthworms peaked when using the cow dung as bait under the following conditions：temperature,25 °C;inoculum density,12 earthworms per 90 g dry bait;humidity,65%;and ratio of C/N,19.4.Range analysis showed that the temperature had greater effects on the total cocoon production of earthworms.