Ag(Ⅰ)-溶菌酶相互作用的光谱学研究

Spectroscopic Study on the Interaction between Ag(Ⅰ) and Lysozyme

利用紫外可见光谱和红外光谱研究了Ag(Ⅰ)与溶菌酶之间的相互作用和酶微观结构的变化.紫外可见光谱测试表明Ag+与溶菌酶肽链上的C=O存在相互作用,红外光谱测试表明Ag+与溶菌酶结合位点可能为—OH和—NH基团,利用二阶导、自退卷积和谱线拟合技术对溶菌酶红外谱图的酰胺Ⅰ带进行处理并推测其二级结构变化,结果表明:α-螺旋二级结构含量降低,β-折叠二级结构含量增加,与Ag+作用后,溶菌酶的活性没有明显改变.

The interaction of Ag（Ⅰ） and lysozyme has been studied with ultra-visible spectrum,and Fourier transform infrared（FT-IR） spectrum.The results of UV-vis spectra show that Ag（Ⅰ） can react with CO groups of peptide chains of lysozyme.The spectra and data of FT-IR indicate lysozyme can provide multiple binding sites including —OH、—NH for Ag（Ⅰ）.The secondary structure contents of lysozyme,including α-helix、β-sheet、β-turn and random coil are calculated from the analysis of amide Ⅰ band of FT-IR by second derivative,Fourier self-deconvolution（FSD） and curve-fitting method.Fitting results show that the content of α-helix decreases while the content of β-sheet increases.Activity of lysozyme does not obviously reduce after reacting with Ag（Ⅰ）.