款冬花总黄酮提取工艺及数学模拟研究

Study on ultrasonic extraction process of flavonids from Tussilago farfara

目的：研究以款冬花为原料、乙醇为溶剂,超声提取黄酮类化合物工艺过程的最优条件。方法：以总黄酮得率为评价指标,在单因素试验基础上通过响应面试验优化乙醇体积分数、超声时间和温度等为考察因素。结果：款冬花总黄酮的最佳超声提取工艺：乙醇体积分数为65%,提取温度为73℃,液料比为10 mL/g,提取时间为47 min,提取率10.74%。结论：超声提取款冬花总黄酮工艺具有简易可行、提取速度快等优点,为进一步的工艺开发有较好的参考价值。

Objective： To study the technologies of separating flavonoids from Tussilago farfara with ultrasonic wave.Methods： On the basis of single-factor test,the response surface analysis were employed to determine the optimum technological condition.Results： The optimum extract method of the flavonids from Tussilago farfara was determined： the concentration of ethanol 65%,the extracted temperature 73 ℃,the ratio of solvent tomaterial 10（mL/g）,the extracted time 47 min.The flavonids extraction yield was 10.74%.Conclusion： The result showsthat the ultrasonic method is time-saving and easily,compared with the traditional methods.The findings provide someinformation for the further researches of flavonids from Tussilago farfara.