潜阳化瘀方抗视网膜静脉阻塞新生血管的实验研究

Explore the inhibiting new vessels effect of Checking Exuberance of Yang and Removing Blood Stasis Formula

目的:探讨潜阳化瘀方对有色家兔抗视网膜静脉阻塞新生血管的作用。方法:将家兔随机分为A(空白组)、B(模型组)、C(羚角钩藤汤组)、D(复方血栓通组)、E(潜阳化瘀方组)5组,每组12只。以氩激光照射视网膜静脉从而阻塞主干静脉的方法造模,分别于进行眼底照相及FFA和免疫组织化学检测,对VEGF(血管内皮生长因子)和bFGF(碱性成纤维细胞生长因子)进行计算机图像测定分析。结果:14 d时,潜阳化瘀方组VEGF在血管壁视网膜节细胞层,内丛状层阳性表达颗粒减少,灰度值与同时间B组比较,差异有统计学意义(P<0.05),该组bFGF在内核层,内丛状层的阳性表达颗粒减少,与同时间B组比较,差异有统计学意义(P<0.05)。28 d时,羚角钩藤方组VEGF表达阳性,并可见较大的阳性bFGF表达颗粒,与模型组比较无明显差异(P>0.05);潜阳组VEGF表达呈阴性,其灰度值与模型组比较,差异有统计学意义(P<0.05),该组bFGF表达亦呈阴性。结论:潜阳化瘀方能够抑制VEGF和bFGF,从而抑制视网膜静脉阻塞(RVO)新生血管形成。

Objective Explore the inhibiting new vessels effect of Checking Exuberance of Yang and Removing Blood Stasis Formula.Methods Divide rabbits into A（control group）,B（model group）,C（Ling jiao Gouteng group）,D（Xue shuan tong group）,E（Qian yang Hua yu group）,five groups,each group 12 rabbits.By argon laser retinal vein occlusion trunk vein approach to modeling,on the day of modelling and 1d,14d and 28d after that,take eye-ground photography and FFA test.Use the parafin section of rabbit＇s eyeball to conduct immunol histochemistry to test VEGF and bFGF and analyze these two target by computer.Results After 14 days,the expression of VEGF in retina strata ganglionare and inner molecular layer of blood vessels walls in checking exuberance of yang group decreased,which had statistical significance compared with the group B.The grey level was182.15±9.67,and AOD was 0.443±0.05,bFGF of retina strata ganglionare and inner molecular layer in group B decreased,which had statistical significance.After 28 days,Ling jiao Gou teng group＇s VEGF expressed positive,and bigger bFGF granule could be seen.Compared with modelling group,there was no significant difference.Checking exuberance of yang group＇s VEGF was negative,and its grey level was 196.53±7.08,AOD was 0.380±0.039,which had stasitical significance compared with model group（P0.05）.bEGF in that group was also negative.Conclusions Checking Exuberance of Yang andRemoving Blood Stasis Formula has the effect of inhibiting VEGF and bFGF,consequently inhibiting the new vessels due to retinal vein obstruction（RVO）.