两种梅毒检测方法的比较

Comparison of the two methods on examing the syphilis

目的用CHEMCLIN(R)600全自动化学免疫发光仪(CLIA)法和梅毒螺旋体明胶颗粒凝集试验(TPPA)分别检测梅毒螺旋体特异性抗体,并对两种方法进行方法学评价。方法将150例门诊确诊为梅毒患者的血清标本分别使用CLIA法与TPPA检测梅毒螺旋体特异性抗体,观察二者的敏感度及特异性。结果 150例血清标本中,CLIA法检测阳性148例,阳性率为98.67%;TPPA法检测阳性144例,阳性率为96.00%。结论 CLIA法检测梅毒螺旋体特异性抗体具有操作简便,结果易于保存,敏感度优于TPPA法等特点,越来越广泛地得到临床的认可和应用。

Objective To evaluate two methodologies of CLIA and TPPA on examing the treponema pallidum specific antibody.Methods The serum specimens from 150 patients with syphilis were collected,then we tested the treponema pallidum specific antibody by CLIA and TPPA,respectively,and the sensitivity and specificity of the two methods on examing syphilis were observed.Results 148 out of 150 samples were tested positive by CLIA method,with a positive rate of 98.67%.While 144 out of 150 samples were tested positive by TPPA method,with a positive rate of 96.00%.Conclusion Using the CLIA method to test the treponema pallidum specific antibody is simple,and the result is easy to preserve and the sensitivity is superior to the TPPA method,and it is widely recognized and applied.