信号转导和转录激活因子5在小鼠CD4^+CD25^+调节性T细胞中的表达

Expression of transcription factor STAT5 in mouse CD4^+ CD25^+ regulatory T cells

目的:观察信号转导和转录激活因子5(STAT5)在小鼠CD4^+CD25^+调节性T细胞中的表达情况。方法:免疫磁珠法分离C57BL/6J小鼠脾脏中的CD4^+CD25^+调节性T细胞,共聚焦荧光法检测细胞内STAT5的分布并进行初步定位,进一步采用Western blot技术从蛋白水平检测细胞内STAT5的表达。结果:激光共聚焦显微镜成像显示STAT5蛋白主要存在于CD4^+CD25^+调节性T细胞胞浆中,细胞总蛋白Western blot检测显示出清楚的STAT5和磷酸化STAT5条带,分子质量分别为92 kD和94 kD。结论:STAT5在小鼠CD4^+CD25^+调节性T细胞内存在表达且主要为胞浆表达。

Objective： To investigate the expression of transcription factor signal transducers and activators of transcription （STAT） 5 in mouse CD4＋ CD25＋ regulatory T cells. Methods： CD4＋ CD25＋ regulatory T cells from the spleen of C57BL/6J mice were isolated by means of MACS microbeads, and then were evaluated with Western blot test and confocal microscopy analysis for STAT5 expression. Results： Confocal microscopy analysis showed that mouse CD4＋ CD25＋ regulatory T cells did express STATS protein in its cytoplasm. Through Western blotting it was revealed distinct bands of STAT5 and p-STAT5, and their molecular weight was 92 kD and 94 kD respectively. Conclusion： STAT5 protein can be markedly expressed in mouse CD4＋ CD25＋ regulatory T cells, and it is mainly located in the cytoplasm of these cells.