转化生长因子β1、基质金属蛋白酶2和9在唾液腺腺样囊性癌中的表达

Expression and clinical significance of transforming growth factor-β1, matrix metalloproteinase-2 and matrix metalloproteinase-9 in salivary adenoid cystic carcinoma

目的研究人唾液腺腺样囊性癌(SACC)组织中转化生长因子β1(TGF-β1)、基质金属蛋白酶2(MMP-2)和MMP-9的表达情况,探讨它们的相关性及与临床病理特征的关系。方法应用免疫组织化学SP二步法检测27例SACC组织标本(癌组)和15例正常唾液腺组织标本(对照组)中TGF-β1、MMP-2和MMP-9的表达情况。所有数据采用SPSS17.0统计软件包进行统计学分析。结果 TGF-β1、MMP-2和MMP-9在SACC组中的阳性表达率(分别为77.78%、81.49%和77.78%)均明显高于在正常唾液腺对照组中的阳性表达率(分别为40%、26.67%和26.67%),差异有统计学意义(均为P<0.01)。SACC组织中TGF-β1、MMP-2和MMP-9在Ⅲ～Ⅳ期组的阳性表达率(均为94.44%)明显高于Ⅰ～Ⅱ期组(分别为44.44%、55.56%和44.44%),差异有统计学意义(分别为P<0.01、P<0.05和P<0.01);而三者的表达与患者的性别、年龄、原发肿瘤部位、病理分型以及有无神经侵袭、复发和转移均无相关性(均为P>0.05);TGF-β1与MMP-2和MMP-9的表达呈正相关(分别为r=0.771、P<0.001和r=0.805,P<0.001)。结论 TGF-β1、MMP-2和MMP-9的高表达与SACC的临床进展密切相关。TGF-β1可能通过调控MMP-2和MMP-9共同参于SACC的发生、发展、侵袭及转移过程。

Objective To investigate the expression of transforming growth factor-β1 （TGF-β1）, matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （MMP-9） in human salivary adenoid cystic carcinoma （SACC） tissues, and to explore their relation with clinicopathological features. Methods Two-step immunochemistry staining method was employed to detect protein expression of TGF-β1, MMP-2 and MMP-9 in specimens from 27 ceases with human SACC and 15 cases with normal human salivary epithelial tissues. All data were analyzedby SPSS 17.0 software package. Results Positive expression rates of TGF-β1, MMP-2 and MMP-9 was 77.78%, 81.49% and 77.78% in SACC tissues and 40%, 26.67% and 26.67% in normal salivary tissues respectively. The expression of TGF-β1, MMP-2 and MMP-9 in SACC tissues increased compared with normal salivary tissues respectively （P 0.01）. Positive expression rates of TGF-β1, MMP-2 and MMP-9 at the third and forth phases （94.44%, 94.44%, 94.44%） was higher than that at the first and second phases （44.44%, 55.56% and 44.44%） in SACC. The differences were statistically significant （P 0.01, P 0.05 and P 0.01 respectively）; their expression was not correlated with patient′s gender, age, region of primary tumor, pathological typing, and nerve invasion, local recurrence as well as distant metastasis （P 0.05）; TGF-β1 had a positive relationship with MMP-2 and MMP-9 in protein expression in SACC （r=0.771, P 0.001 and r=0.805, P 0.001 respectively）. Conclusions Increased expression of TGF-β1, MMP-2 and MMP-9 may be associated with clinical progression of SACC, and TGF-β1 may participate in the process of SACC initiation, progression, invasion and metastasis through regulating MMP-2 and MMP-9.