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胃癌DAPK1基因启动子甲基化状况及表达的研究

Research on Abnormal Methylation of DAPK1 Gene in Human Gastric Cancer
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摘要 目的对DAPK1基因甲基化状态、表达进行研究,探讨基因甲基化在胃癌发病中的作用。方法用半定量RT-PCR方法研究胃癌及癌旁组织中DAPK1基因的表达,用甲基化特异性PCR(MSP)对胃癌及癌旁组织中DAPK1基因启动子区甲基化情况进行研究。结果 DAPK1有16例表达下调,6例表达上调,基因在癌旁组织的mRNA表达明显高于癌组织,具有统计学意义(P=0.465)。DAPK1在胃癌组织及癌旁组织中甲基化率均为100%,无统计学意义(P>0.05)。结论胃癌组织中DAPK1基因表达下降与该基因甲基化无明确相关性。 Objective To study the situation of DAPK1 gene methylation and the expression of DAPK1 in human gatric cancer tissues.Methods The methylation of DAPK1 was detected by MSP,and the expression of DAPK1 was detected by RT-PCR.Results The low expression of DAPK1＇s mRNA is 16/35（P=0.465）.The rate of DAPK1 gene methylation in human gastric cancer tissues and cancer-adjacent tissues is both 100%（P0.05）.Conclusion The low expression of DAPK1 in gastric cancer tissues isn＇t related with the gene promoter＇s methylation.
作者 王贺玲 张健 李岩
机构地区 沈阳市第一人民医院消化内科 中国医科大学第二附属医院消化内科
出处 《中外医疗》 2011年第11期35-36,38,共3页 China & Foreign Medical Treatment
关键词 DAPKI基因 胃癌 甲基化 DAPK1 gene Gastric cancer Methylation
分类号 R735.2 [医药卫生—肿瘤]
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参考文献11

  • 1M Esteller.Epigenetics provides a new generation of oncogenes and tumour-suppressor genes[J].British Journal of Cancer,2006,94:179～183.
  • 2Yamada Y,Jackson-Grusby L,Linhart H,et al.Opposing effects of DNA hypomethylation on intestinal and liver carcinogenesis[J].Proc Natl Acad Sci USA,102:13580～13585.
  • 3Beier V,Mund C,Hoheisel JD.Monitoring methylation changes in cancer[J].Adv Biochem Eng Biotechnol,2007,104:1～11.
  • 4Chim CS,Liang R,Leung MH,et al.Aberrant gene methylation implicated in the progression of monoclonal gammopathy of undetermined significance to multiple myeloma[J].J Clin Pathol,2007,60(1):104～106.
  • 5陆嵘,房静远.表观遗传修饰与肿瘤[J].生命科学,2006,18(1):10-14. 被引量：29
  • 6张永彪,褚嘉祐.表观遗传学与人类疾病的研究进展[J].遗传,2005,27(3):466-472. 被引量：49
  • 7Tanaka T,Bai T,Yukawa K,et al.Radiation-induced cell death is independent of the apoptotie signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells[J].Oncol Rep,2005,14(4):949～955.
  • 8Shang T,Joseph J,Hillard CJ,et al.Death-associated protein kinase as a sensor of mitochondrial membrane potential:role of lysosome in mitoehondrial toxin-induced cell death[J].J Biol Chem,2005,280(41):34644～34653.
  • 9Sobin LH,Fleming ID.TNM classification of malignant tumors,fifth edition (1997).Union Internationale Contre le Cancer and the American Joint Committee on Cancer[J].Cancer,1997,80:1803～1804.
  • 10Zhang WF,Chen JQ,Shan JX,et al.Indication of radical surgery (R2,R3) based upon the pattern of lymph node metastasis from gastric cancer[J].Zhonghua Zhongliu Zazhi,1987,9:286～289.

二级参考文献146

  • 1Alvarez-Venegas R, Avramova Z. Methylation patterns of histone H3 Lys 4, Lys 9 and Lys 27 in transcriptionally active and inactive Arabidopsis genes and in atxl mutants.Nucleic Acids Res, 2005, 33(16):5199-5207.
  • 2Baylin S B. Tying it all together: epigenetics, genetics, cell cycle and cancer. Science, 1997, 277(5334):1948-1949.
  • 3Kanai Y, Ushijima S, Kondo Y, et al. DNA methyltransferase expression and DNA methylation of CpG island and pericentromeric satellite region in human colorectal and stomach cancers, Int J Cancer, 2001, 91(2): 205-212.
  • 4Tycko B. Epigenetic gene silencing in cancer. J Clin Invest,2000, 105(4): 401-407.
  • 5Nephew K P, Huang T H. Epigenetic gene silencing in cancer initiation and progression. Cancer Lett, 2003, 190(2):125-133.
  • 6Linggi B E, Brandt S J, Sun Z W, et al. Translating the histone code into leukemia. J Cell Biochem, 2005, 96(5): 938-950.
  • 7Yang X J, Ogryzko V V, Nishikawa J, et al. A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Nature, 1996, 382(6589): 319-324.
  • 8Loprevite M, Tiseo M, Grossi F, et al. In vitro study of CI994, a histone deacetylase inhibitor, in non-small cell lung cancer cell lines. Oncol Res, 2005, 15(1): 39-48.
  • 9Mahadevan L C, Willis A C, Barratt M J. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell,1991, 65(5): 775-783.
  • 10Nowak S J, Corces V G. Phosphorylation of histone H3: a balancing act between chromosome condensation and transcriptional activation. Trends Genet, 2004, 20(4): 214-220.

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中外医疗
2011年 第11期

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