新城疫病毒诱导体外培养喉癌细胞凋亡

Apoptosis in human laryngeal neoplasm cell line induced by Newcastle disease virus in vitro

目的为探讨新城疫病毒(Newcastle Disease Virus,NDV)对体外培养喉癌细胞的杀伤作用及其与喉癌细胞凋亡的关系。方法本实验利用新城疫病毒弱毒疫苗株LaSota接种于体外培养的喉癌细胞系(Hep-2),不同时间后,在光镜、电镜下对细胞形态、结构进行观察,并收集细胞采用流式细胞术对细胞凋亡情况进行了检测。结果接毒后48小时,开始出现部分Hep-2细胞脱壁;60h后出现细胞溶解、聚集,部分死亡细胞悬浮于培养液中;电镜下可见细胞间大量堆积的病毒粒子,细胞核出现染色质浓缩、集边、核膜扩张、胞浆浓缩等变化;还可见完整细胞膜包围的凋亡小体出现。另外,在接毒后48h流式细胞仪的DNA直方图上,出现细胞凋亡峰,凋亡细胞百分数随着接毒时间的延长而增加。结论新城疫病毒感染可大量杀伤体外培养的喉癌细胞,并可诱导其发生凋亡性坏死。

Objective Investigate the anti-tumor effect of La Sota strain of Newcastle disease virus（NDV） on human tumor cell.Methods The Hep-2 cell was infected with La Sota strain of NDV,and the changes of cell structure were observed under optical and electronic microscope.Results Laryngeal neoplasm cells secession,chomatin condensation,and apoptotic bodies were found 48h post-infection.Flow cytometry analysis shows that：33.7%of the Hep-2 cell were apoptotic cells at 48h post-infection.Conclusion Consequently,it is clear that NDV infection can kill the Hep-2 cell efficiently and induce apoptosis in it.