染色体3q21.3常见变异rs10934853单核苷酸多态性与前列腺癌风险的关联

Association between SNP rs10934853 of chromosome 3q21.3 and risk of prostate cancer

目的探讨3q21.3内常见变异rs10934853单核苷酸多态性与中国人前列腺癌发病风险的关系,并探讨其与临床相关危险因素的相互作用。方法应用病例对照方法,选取90例前列腺癌患者以及年龄、性别匹配的109例正常对照人群为研究对象,采用聚合酶链反应-高分辨熔解曲线(PCR-HRM)技术结合测序验证法检测其3q21.3内rs10934853单核苷酸多态性的分布情况。结果①前列腺癌组中AA,CC,AC基因型分别为19例(21.1%),34例(37.8%),37例(41.1%);正常对照组中AA,CC,AC基因型分别为24例(22.0%),34例(31.2%),51例(46.8%);两组间基因型频率和等位基因频率的分布无显著性差异(P>0.05)。②Rs10934853单核苷酸多态性的不同基因型与前列腺癌患者的年龄,BMI指数,Gleason评分以及PSA浓度指标间均无显著相关性(P>0.05)。结论 Rs10934853单核苷酸多态性与中国人前列腺癌的发生无明显相关性,可能与中国人前列腺癌发病风险无关。

Objective To investigate the association between the common variant SNP rs10934853 on chromosome 3q21.3 and risk of prostate cancer,and explore its interaction with related risk factors of prostate cancer.Methods A case-control method was performed,consisting of 90 patients with prostate cancer and 109 age and gender matched cancer-free healthy controls.Polymerase chain reaction-high resolution melting curve（PCR-HRM） combined with gene sequencing methods were used to determine the distribution of allele and genotype frenquencies of the SNP rs10934853 on chromosome 3q21.3.Results ①In 90 patients with prostate cancer,the AA,CC,AC genotypes were found in 19 patients（21.1%）,34（37.8%）,37 patients（41.1%） ;and in 109 healthy controls,the AA,CC,AC genotypes were found in 24 patients（22.0%）,34 patients（31.2%）,51 patients（46.8%） respectively.There was no significant difference of the distribution of genotype and allele frequency between the two groups（P 0.05）.②The different genotypes of rs10934853 on chromosome 3q21.3 were not associated with age,BMI,Gleason score and PSA levels of prostate cancer patients（P 0.05）.Conclusion There is no obvious association between SNP rs10934853 on chromosome 3q21.3 with prostate cancer in Chinese.It may not be the genetic risk factor of prostate cancer.