核因子-κB和细胞间黏附因子-1的水平变化与胎膜早破的关系

Clinical significance of nuclear factor-κB and intercellular adhesion molecule-1 in patients with preterm premature rupture of membranes

目的:探讨母血、羊水、脐血中核因子-κBp65(NF-κB p65)及细胞间黏附因子-1(IACM-1)的水平变化与胎膜早破发病的关系。方法:采用双抗体夹心酶联免疫吸附法,动态检测62例胎膜早破患者(胎膜早破组)及46例正常孕妇(对照组)的母血、羊水、脐血中NF-κB p65及IACM-1的水平,同时进行胎膜组织的病理检查,对合并绒毛膜羊膜炎的患者也进行相应的NF-κB p65及IACM-1的水平检测。结果:PROM组母血、羊水、脐血中NF-κB p65和IACM-1的含量均高于对照组(P<0.05)。绒毛膜羊膜炎患者的母血、脐血、羊水中NF-κB p65水平明显高于非绒毛膜羊膜炎患者,两者比较,差异有统计学意义(P<0.05)。结论:NF-κB p65及IACM-1的水平检测可作为一种新的生物学标志物用于胎膜早破并绒毛膜羊膜炎感染的诊断。

Objective Investigate the clinical significance of nuclear factor-κB p65（NF-κB p65） and intercellular adhesion molecule-1（IACM-1） in patients with preterm premature rupture of membranes（PROM） and chorioamnionitis.Methods The levels of NF-κB p65 and IACM-1 were detected by enzyme linked immunosorbent assay in maternal serum,amniotic fluid,umbilical cord serum of 62 pregnant women with PROM and 46 women with normal pregnancy.Every embryolemma was histopathologically confirmed after delivery.Results The levels of NF-κB p65 and IACM-1 in maternal serum,amniotic fluid,umbilical cord serum of PROM were respectively significantly higher in patients with PROM than those of the control group（P0.05）.The levels of NF-κB p65 and IACM-1 in maternal serum,amniotic fluid,umbilical cord serum in chorioamnionitiswere higher than those in non-chorioamnionitis（P0.05）.Conclusion The levels of NF-κB and IACM-1 concent rations in maternal serum,amniotic fluid,and umbilical cord serum is a useful biomarker for identifying the chorioamnionitis and predicting premature rupture of membrance.