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环形泰勒虫suAT1基因的克隆及序列分析

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摘要 以环形泰勒虫兰州株基因组为模板,经PCR扩增获得了suAT1的部分基因,将该基因克隆到pMD20-T载体,对重组质粒进行PCR和双酶切鉴定及序列测定。结果表明:该基因的长度为1 377 bp,编码了459个氨基酸。同源性分析结果显示:克隆序列与GenBank收录的环形泰勒虫参考核苷酸序列同源性为98.35%,氨基酸同源性为97.13%,说明环形泰勒虫兰州株的suAT1与GenBank上公布的序列同源性很高,进一步说明了suAT1基因存在很强的保守性。
出处 《江苏农业科学》 CSCD 北大核心 2011年第2期40-42,共3页 Jiangsu Agricultural Sciences
基金 国家肉牛牦牛产业技术体系资金(编号:nycytx-38) 中央级公益性科研院所基本科研业务费专项(编号:BRF100402)
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