6Meyermand H,Morreel K,Lapierre C,et al.Modifications in lignin and accumulation of phenolic glucosides in popular xylem upon dowenregulation of caffeoyl A O-Methyltransferase,an enzyme involved in lignin biosynthesis[J].The Journal of Biological Chemistry,2000,275(47):36899-36909.
7Zhong R,Morrison W H,Negrel J.Dual methylationpathways in hignin biosynthesis[J].Plant Cell,1998,10:2003-2046.
8Guo D J,Chen F,Inue K,et al.Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfafa:impacts on legnin structure and Implications for the biosynthesis of Gand S lignin[J].The Plant Cell,2001,13:73-88.
9Hu W J,Harding S A,Lung J.Repression of lignin biosynthesis promotes cellulose accumulation and,growth in transgenic trees[J].Nat Biotech,1999,17:808-812.
2Halpin C. Manipulation of lignin quality by down-regulation of cinnamyl alcohol dehydrogenase. Plant J, 1994,6:339-350.
3Wei J H, Zhao H Y, Zhang J Y, et al. Cloning of cDNA encoding CCoAOMT from Populus tomentosa and down-regulation of lignin content in transgenic plant expressing antisense gene. Aeta Botanica Sinica, 2001,43(11): 1 179-1 183.
4Elkind Y. Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylaLanine ammonia-lyase gene. Proc Nail Acad Sci USA, 1990,87:9 057-9 061.
5Sewalt V J H, Ni W, Blount J W. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia lyase or cinnamate 4-hydrelase. Plant Physiology, 1997,115:41-50.
6Piquemal J, Lapierre C, Myron K. Down-regulation of cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plants J, 1998,13:71-83.
7Kajita S, Katayama Y, Omori S. Alterations in the biosynthesis of lignin in transgenlc plants with chimeric genes for 4-coumarate: coenzyme A ligase. Plant Cell Physiol, 1996,37:957-965.
8Hu W J, Harding S A, Lung J. Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nat Biotech, 1999,17:808-812.
9An G. Binary Ti vectors for plant transformation and promoter analysis. Meth Enzym, 1997, 153 : 293-305.
10Richard A J. β-glucuronidase from Escherichia coli as agene-fusion marker. Proc Natl Acad Sci USA, 1986,83:8 447-8 451.
5ULRICH Z, STEFAN H. Role of heat shock proteins in protection from and pathogenesis of infectious diseases [ J]. Clinical Microbiology Reviews, 1999, 12(1) :19-39.
6JIN L, ZHU A H, WANG Y, et al. Enhanced immunogenicity of peptide P277 by heat shock protein HSP65 vector carrying tandem repeats of P277 to prevent type 1 diabetes in NOD mice [J]. Exp Clin Endocrinol Diabetes, 2008, 116 : 1-8.
7JIN L, WANG Y, XIONG Q, et al. Long-lasting specific antibodies against P277 induced by mucosaladministration of P277 repeat sequences carried by Hsp65 in the absence of adjuvant [ J]. Vaccine, 2007, 25: 2043-2050.
8王关林,方宏筠.植物基因工程[M].2版.北京,科学出版社,1998:744.
9NOBUYUKI M. Recombinant protein expression in Nicotiana [ J ]. Methods Mol Biol, 2011, 7(1) :199-219.
10LI H Y, CHYE M L. Use of GFP to investigate expression of plant-derived vaccines [ J]. Methods in Molecular Biology, 2009, 515 : 275-285.