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水胁迫下转基因水稻抗坏血酸-谷胱甘肽循环系统的变化 被引量:2

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摘要 分析了PEG 6000模拟干旱胁迫条件下共表达GST和CAT1的转基因水稻和非转基因水稻抗氧化系统特别是抗坏血酸-谷胱甘肽循环途径的变化。结果显示,在PEG胁迫下,转基因水稻和非转基因水稻的抗氧化系统尤其是整个抗坏血酸-谷胱甘肽循环系统的变化有显著差异。其中转基因水稻的CAT、APX和MDAR的活性以及抗坏血酸的氧化还原状态在胁迫期间都增加,但非转基因水稻的这些指标只在胁迫5 d时增加。然而,非转基因水稻的GR、SOD和GST的活性则随着水胁迫时间的延长显著增加。此外,在胁迫期间,转基因水稻的DHAR活性和GSH的含量也比非转基因水稻的高,但MDA和H2O2的含量则明显低于非转基因水稻的。这些结果表明,水胁迫下,GST和CAT1在转基因水稻中的共表达对整个抗坏血酸-谷胱甘肽循环系统产生了影响。转基因水稻受水胁迫伤害程度的降低是GST和CAT1与抗坏血酸-谷胱甘肽循环系统协同调控的结果。
出处 《江苏农业科学》 CSCD 北大核心 2011年第2期111-114,共4页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:30671126)
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参考文献32

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