反相-流动注射-阻抑光度法测定药物中铋(Ⅲ)

RP-FI-Inhibitory Photometric Determination of Bismuth(Ⅲ) in Drugs

在pH 7.8的H_3BO_3-KCl-NaO缓冲介质中,铋(Ⅲ)对过氧化氢氧化结晶紫(CV)褪色反应具有灵敏的阻抑作用,从而提出了反相流动注射-阻抑光度法测定铋的方法。结果表明:在590nm最大吸收波长处,铋(Ⅲ)的质量浓度在0.005～0.05 mg·L^(-1)范围以内与其△A呈线性关系,检出限(3S/N)为7.28×10^(-1)mg·L^(-1)。方法用于胃药中铋(Ⅲ)的测定,测得方法的回收率为101.5%,测定值的相对标准偏差(n=11)为0.079%。

It was found that in H3BO3-KCl-NaOH buffer medium of pH 7.8,the oxidative color-fading of crystal violet（CV）by H2O2 was inhibited by the presence of Bi（Ⅲ）.Based on these facts,the determination of Bi（Ⅲ）by reverse phase flow injection inhibitory photometry was proposed.Linear relationship between values ofΔA and mass concentration of Bi（Ⅲ）was obtained in the range of 0.005-0.05 mg·L^-1when measured at the absorption maximum of 590 nm.Detection limit（3S/N）of the method found was 7.28×10^-1mg·L^-1.The proposed method was used in the determination of Bi（Ⅲ）in gastric drug,and values of recovery and RSD（n=11） found were 101.5%and 0.079%respectively.