miR-221和miR-222在胃癌中的表达及临床意义

Expression of miR-221 and miR-222 in Gastric Cancer and its Clinical Significance

[目的]探讨miR-221和miR-222在胃癌中的表达及与临床病理特征的关系。[方法]应用茎环RT-PCR方法检测32例胃癌及癌旁胃黏膜组织中miR-221和miR-222表达,分析miR-221和miR-222表达与胃癌临床病理指标的关系。[结果]胃癌组织miR-221和miR-222表达明显高于对应的正常胃黏膜组织(P<0.05),且胃癌与正常胃黏膜组织中miR-221和miR-222比值也有显著性差异(P=0.05)。miR-222表达与胃癌分化程度相关(P=0.046);miR-221表达与淋巴结转移相关(P=0.013)。[结论]miR-221和miR-222在胃癌中表达明显上调是胃癌的重要标志,但两者在胃癌发生发展的不同阶段,可能发挥不同的效应。

[Purpose] To investigate the expression of miR-221 and miR-222 in gastric cancer and its relationship with clinicopathologic features.[Method] The expression of miR-221 and miR-222 in 32 cases of gastric cancer tissues and their matched non-tumor adjacent tissues was examined by stem-loop RT-PCR.And the correlation with clinicopathologic features was investigated.[Results] The expression of miR-221 and miR-222 in gastric cancer tissues was significantly higher than those in adjacent tissues（P0.05）.The ratio of miR-221/miR-222 expression had significant difference（P=0.05）.The expression of miR-222 was related to differentiation（P=0.046）;the expression of miR-221 was related to lymph node metastasis（P=0.013）.[Conclusion] The expressions of miR-221 and miR-222 in gastric cancer markedly up regulation,they are important markers for gastric cancer,and they may play different effect with different stages in gastric cancer.