Sysmex XT-1800i,XS-800i和Mythic-22检测结果可比性研究

Comparative Study on Result of Blood Cells with Hematological Analyzers of Sysmex XT-1800i,Sysmex XS-800i and Mythic-22

目的 探讨日本Sysmex XT-1800i,Sysmex XS-800i和瑞士Mythic-22全自动血细胞分析仪对同一份标本测定结果的可比性.方法 以Sysmex XT-1800i为目标检测系统（对比方法）,并对其进行精密度、准确度评价.以Sysmex XS-800i和Mythic-22为实验检测系统（实验方法）.以门诊和部分住院患者新鲜全血进行血常规检测,对WBC及分类、RBC,HGB,HCT和PLT计算相关系数和回归方程,以美国临床实验室修正法规（CLIA＇88）规定的室间质量评价允许误差的1/2为标准,判断不同检测系统WBC,RBC,HGB,HCT和PLT的可比性.结果 Sysmex XT-1800i血细胞分析仪WBC,RBC,HGB,HCT和PLT精密度在允许范围内,质控项目中除Sysmex XS-800i RBC高值外,其余项目在不同检测系统上的测定结果均具有可比性.非质控项目白细胞分类只有嗜碱性粒细胞相关性差,分别为r=0.349和r=0.427.结论 对该室内不同血细胞检测系统定期进行测定结果的可比性分析,以及时发现系统误差,并进行校准,为临床和患者提供准确的可比性报告.

Objective To investigate comparability of the results of the same blood with hematological analyzers of Sysmex XT-1800i,Sysmex XS-800i and Mythic-22. Methods To assess the accuracy,the intra and inter-assay precision of Sysmex XT-1800i. Compared Sysmex XS-800i, Mythic-22 （laboratory method） to sysmex XT-1800i （comparison method ）, items including WBC,RBC,HGB,HCT and PLT,elass of WBC and to obtain the correlation coefficient and the linear equation. E- valuate the system bias of WBC,RBC,HGB,HCT and PLT between laboratory method and comparison method according to the half of CLLA＇88. Results The precision were allowed of Sysmex XT-1800i. Quality control items were comparative,except the high RBC of Sysmex XS-8.00i. The result of Bas were not satisfied （r=0. 349,r=0. 427）. Conclusion It was necessary to examine different hematology with method comparison in time to improve the accurate report for clinic and patient.