红霉素耐药肠球菌ermB基因检测

Detection of ermB Gene in Erythromycin Resistant Enterococcus

目的 研究北京妇产医院临床感染患者分离的肠球菌对红霉素的耐药情况以及耐药基因ermB的分布特点.方法 测定肠球菌的抗生素敏感性及采用PCR法检测红霉素耐药基因ermB.结果 50株肠球菌中,粪肠球菌39株（78%）,屎肠球菌11株（22%）;红霉素耐药率为88%（44株/50株）.在44株红霉素耐药肠球菌中,ermB基因总的携带率为84.09%（37株/44株）.结论 临床标本中分离的红霉素耐药的肠球菌主要耐药基因为ermB基因.

Objective To study the drug resistance and ermB gene of erythromycin resistant Enterococcus. Methods The antimicrobial susceptibility to antibiotics of Enterococcus was determined,and PCR technique was applied to identify ermB gene. Results Among all of the 50 Enterococcus strains,39 isolates were Enterococcus faecalis, 11 isolates were Enterococcus faecium,the resistance rate to erythromycin was 88 % ,the ermB gene carriage rate in erythromycin resistant Enterococcus was 84.09% （37/44）. Conclusion Resistance to erythromycin in Enterococcus isolates was generally mediated via ermB gene.