酶标仪自检方法的建立和应用评价

Establishment and Application of a Self-calibrating Approach for Microplate Readers

目的 建立酶标仪的自检方法,检定酶标仪的各项性能指标.方法 参照〈中华人民共和国国家计量检定规程-酶标分析仪〉并利用分光光度计,对Multiskan MK3酶标仪各项性能指标进行检定.结果 Multiskan MK3酶标仪示值稳定性（0.001A）、波长示值误差（-1.0 nm）、波长重复性（0 nm）、吸光度示值误差（0.006 A）、吸光度重复性（0.17%）、灵敏度（0.018 L/mg）、通道差异（0.005A）及线性（1.88%）均检定合格.结论 具有性能优良分光光度计的临床实验室可进行酶标仪的自检.

Objective To establish a self-calibration method that monitors and corrects multiple performance indexes for microplate readers in a clinical setting. Methods According to the People＇s Republic of China national metrologieal verification regulations for ELISA analyzer, the performance status of a microplate reader Multiskan MK3 was checked by a spectrophotometer. Results Fore Multiskan MK3 microplate reader,readout stability （0. 001A）,wavelength error （-1.0 nm ）, wavelength repeatability （ 0nm ）, absorbance error （ 0. 006A ）, Absorbance repeatability （0. 17%）, sensitivity （0. 018L/mg）,channel difference （0. 005A） and linear （1. 88% ）. These results were in line with those required by the regulation. Conclusion Clinical laboratories that had advanced spectrophotometers may be able to perform self-calibration of microplate readers by following the method described above.