血清葡萄糖线性评价及其校正的临床研究

The Clinical Study of Linearity Evaluation and Related Correction for Serum Glucose

目的评价血清葡萄糖(GLU)试剂厂家提供的线性范围,运用改良Doumas法重新设定适合本实验室的线性范围。方法用迈瑞BS-300全自动生化分析仪检测高浓度血清葡萄糖,评价厂家提供的线性范围;用低值和高值血清葡萄糖标本配制成系列浓度的样本进行检测,所得数据进行统计分析,运用改良Doumas法重新设定线性范围;运用新的线性范围后对高浓度血糖进行稀释验证试验。结果经校正后血清葡萄糖的线性范围为(0.33～32.4)mmol/L,与试剂厂家提供的线性范围(0～38.9)mmol/L不一致。结论对定量监测项目均应评价及校正其线性范围,防止线性以外的错误结果,从根本上确保检测结果的准确性。

Objective To evaluate the linear range of serum glucose （GLU） reagent provided by the manufacturer, and use modified Doumas method to reset the linear range for our laboratory. Methods With Mindray BS-300 automatic biochemical analyzer,high con- centration serum glucose was test to evaluate the linear range provided by the manufacturer,Then dilute with low and high values of serum glucose concentrations of samples into a series of samples to test, and statistically analysed. The modified Doumas method was used to reset linear range. In the new linear range,high concentrations glucose was diluted to verify it. Results The corrected results of the linear range of serum glncose was （0.33mmol/L- 32.4mmol/L）,which was not consistent with what was provide by the manufacturer （0- 38.9mmol/L）. Cortelusiort Quantitative test item should be evaluated and corrected the linear range,to prevent erroneous results over linearity,essentially to ensure the accuracy of test results.