血必净注射液对脓毒症大鼠肺损伤的保护作用

Protective Effects of Xuebijing Injection on Lung Injury in Rats With Sepsis

目的:探讨血必净注射液对脓毒症大鼠肺损伤的保护作用及机制。方法:采用盲肠结扎穿孔法(CLP)建立脓毒症模型,雄性Wistar大鼠80只,随机分成4组:正常对照组(N组,8只);假手术组(S组,8只);脓毒症组(CLP)(C组,32只)和血必净治疗组(X组,32只),C组和X组分别于CLP后经大鼠颈静脉注射生理盐水或血必净注射液2ml/kg,分别于CLP后3、6、12和24h各处死8只动物,取肺组织,比色法检测丙二醛(MDA)和髓过氧化物酶(MPO),酶联免疫吸附法检测血清肿瘤坏死因子α(TNF-α)水平。结果:脓毒症时各时间点肺组织MDA、MPO和血清TNF-α浓度较对照组显著升高,而血必净治疗后则有明显下降(P<0.01)。结论:血必净注射液可减轻脓毒症时的肺损伤,其机制可能与减少肺组织MDA、MPO和血清TNF-α表达有关。

Objective：To investigate the protective effects and mechanisms of the integrated traditional Chinese medicine Xuebijing injection on lung injury of rats with sepsis.Methods：Sepsis was established in rats by cecum ligation and puncture（CLP）.Eighty male Wistar rats were randomly divided into normal control group（group N,n=8）,sham operation group（group S,n=8）,CLP model group（group C,n=32）,Xuebijing treated group（group X,n=32）.Rats in group C and X were given normal saline or Xuebijing injection with a dose of 2 ml/kg intravenously after CLP models were established respectively.Then 8 rats were sacrificed at 3,6,12,and 24 hours after CLP.The lungs were removed for detecting malondialdehyde（MDA）and myeloperoxidase（MPO）;serum tumor necrosis factor alpha（TNF-α）was also examined.Results：The rats with sepsis demonstrated an increase in MDA,MPO activity of the lung tissue and TNF-α activity of serum.In contrast,the rats after Xuebijing injection treatment exhibited a significant lowering in MDA,MPO and TNF-α activity.Conclusions：Xuebijing injection treatment might ameliorated lung injury by down-regulating the expressions of MDA,MPO and serum TNF-α.