强化胰岛素治疗对糖尿病大鼠胰岛β细胞凋亡蛋白bcl-2和bax的影响

The effects of intensive insulin treatment on islet β cell apoptosis associated protein bcl-2 and bax in type 2 diabetic rats

目的观察强化胰岛素治疗对糖尿病大鼠胰岛β细胞凋亡相关蛋白bcl-2和bax的影响。方法将36只Wistar大鼠随机分为对照组和高脂组，对照组给予基础饲料喂养，高脂组给予脂肪乳灌胃＋基础饲料喂养10d，然后给高脂组大鼠腹腔注射链脲佐菌素，3d后再将高脂组大鼠随机分为2个亚组，即糖尿病对照组和糖尿病治疗组，疗程4周。在脂肪乳灌胃第10天、注射STZ第3天和治疗4周末测大鼠各项指标；实验结束时用免疫组化法测大鼠胰岛β细胞凋亡相关蛋白bcl-2和bax的表达。结果大鼠在强化胰岛素治疗4周末，与糖尿病对照组相比，糖尿病治疗组的bcl-2蛋白表达升高，bax蛋白表达降低，两组差异均有统计学意义（均P〈0．05）。结论强化胰岛素治疗可增强2型糖尿病大鼠胰岛β细胞bcl-2蛋白表达，降低bax蛋白表达，减轻2型糖尿病大鼠胰岛β细胞凋亡。

Objective To observe the effects of intensive insulin treatment on islet β cell apoptosis associated protein bcl-2 and bax in type 2 diabetic rats. Methods 36 Wistar rats were randomly divided into two groups：normal control group and high fat diet group. Rats in normal control group fed by basical feedstuff. Rats in high fat diet group fed by high fat and basical feedstuff. After 10 days,rats in high fat group were injected with STZ. After 3 days, rats in high fat group were randomly divided into two groups：diabetes control group and insulin treatment group. The course of treatment was 4 weeks. After 10 days by fat milk intragastric administration, after 3 days of STZ injection and after 4 weeks treatment, each index was measured. After experiment, pancreatic tissue bel-2 and bax were detected through method. Results After 4 weeks intensive insulin treatment,the bcl-2 was significantly increased at（6. 20±2.05 ）% in insulin treatment group than diabetes control group. The bax was significantly decreased at （2.68±1.04 ） % in insulin treatment group than diabetes control group （ P 〈 0.05 ）. Conclusion The method of insulin intensive treatment could increase islet β cell bcl-2 and decrease bax in type2 diabetic rats. Insulin intensive treatment could decrease islet β cell apoptosis.