ErbB4基因micro-RNA靶序列单核苷酸多态性与乳腺癌易感性的关系

Relationship between polymorphism of ErbB4 gene in mirco-RNA binding site and the risk for breast cancer

目的:探讨ErbB4基因micro-RNA靶序列单核苷酸多态性与乳腺癌易感性的关系。方法:采用TaqMan单核苷酸多态(single nucleotide polymorphism,SNP)分型技术检测1509例乳腺癌患者和1517例健康对照者外周血中ErbB4基因micro-RNA靶序列rs1595066及rs16845990位点的基因型。结果:ErbB4基因micro-RNA靶序列rs16845990位点3种基因型在乳腺癌患者和健康对照者间的分布差异无统计学意义(P=0.302);rs1595066位点3种基因型在2组间的分布差异有统计学意义(P=0.045),与GG型相比,杂合型AG及纯合型AA均可显著降低乳腺癌的发病风险,调整后比值比(oddsratios,OR)及其95%可信区间(confidenceintervals,CI)分别为0.81(0.69～0.95)和0.75(0.58～0.96)。分层分析显示,此保护作用在年龄≥55岁、无肿瘤家族史和无乳腺良性疾病史者中更显著。结论:ErbB4基因micro-RNA靶序列rs1595066G>A位点A等位基因可能降低乳腺癌的发病风险。

Objective：To investigate the relationship between single nucleotide polymorphism（SNP） of ErbB4 gene in mirco-RNA binding site and the susceptibility of breast cancer.Methods：Genotypes of ErbB4 gene in mirco-RNA binding site at rs1595066 and rs16845990 were measured using the TaqMan SNP method in 1 509 patients with breast cancer and 1 517 healthy controls.Results：Three kinds of genotype of ErbB4 gene in mirco-RNA binding site at rs1595066 were significantly different between breast cancer patients and the healthy controls（P=0.045）,while there was no difference at rs16845990 site（P=0.302）.The carriers of AG and AA genotypes at rs1595065 site had lower risk of breast cancer than GG genotype [（odds ratio,OR） = 0.81,95%（confidence interval,CI） = 0.69-0.95;OR = 0.75,95% CI = 0.58-0.96].Stratified analysis showed that this protective effect was significant in the subjects aged over 55 years,without a history of benign breast disease and without a family history of cancer.Conclusion：The polymorphism of rs1595066 GA in micro-RNA binding site of ErbB4 gene may be associated with the reduction risk of breast cancer.