实验性脑梗死对大鼠心率变异性的影响

Effect of Experimental Cerebral Infarction on Heart Rate Variability in Rats

目的探讨急性脑梗死对心脏自主神经活性的影响。方法 Wistar大鼠32只随机分为正常组、假手术组和脑梗死组,脑梗死组用线栓法行右侧大脑中动脉阻塞。脑梗死组和假手术组于术前及术后24h作心率变异性(HRV)检测,同时检测正常组HRV,将3组的HRV指标进行比较。实验终点取各组心肌组织检测儿茶酚胺和神经肽Y(NPY),进行组间比较。结果术后24h脑梗死组和正常组、假手术组相比,窦性心搏间期标准差、均方根,总功率谱、高频功率谱(HF)、低频功率谱(LF)降低,差异有统计学意义。3组比较LF/HF和分数维无明显差异。脑梗死组心肌组织去甲肾上腺素(NA)和NPY高于正常组和假手术组。结论脑梗死引起心脏自主神经总活性降低、自主神经功能受损,自主神经末梢去甲肾上腺素和NPY的异常分泌可能是重要的原因。

Objective To investigate the effect of acute cerebral infarction on cardiac autonomic nervous activity.Methods A total of 32 Wistar rats were divided into normal group,sham operation group and infarction group by random.Experimental cerebral infarction in Wistar rats was induced by intraluminal occlusion of middle cerebral artery.About 24 hours after the occlusion or 24 hours after sham operation,the heart rate variability（HRV） sequences were measured,and the HRV values in the three groups were compared.The levels of catecholamine and neuropeptide（NPY） in myocardium were measured.Results At the 24th hour after the occlusion,the standard deviation and root mean square standard deviation of R-R interval,the total power,high frequency（HF） and low frequency（LF） in infarction group were lower than those in normal and sham operation group.LF/HF and fractal dimension did not differ much among the three groups.The levels of noradrenaline and NPY in myocardium in infarction group were higher than those in the other groups.Conclusion It is suggested that acute cerebral infarction may cause the decrease of autonomic nervous activity and damage of the autonomic nervous function;the abnormal secretion of noradrenalin in autonomic nerve ending and NPY may be the important reasons.