Cyclin E表达对于原代乳腺癌细胞侵袭性的影响

Correlation between expression of Cyclin E in primary breast cancer cells and Invasion of breast cancer cells

目的:从细胞学角度探讨Cycli E表达水平与原代乳腺癌细胞侵袭之间的关系。方法:手术中无菌条件下切取乳腺癌组织,制备原代乳腺癌细胞悬液,运用Transwell小室进行原代乳腺癌细胞侵袭力实验;获取具有不同侵袭力的原代乳腺癌细胞,再用免疫荧光技术检测不同侵袭能力的原代乳腺癌细胞cyclin E的表达水平,研究两者相关性。结果:具有不同侵袭力的乳腺癌细胞表达不同强度的Cyclin E蛋白,分别计数5个高倍视野,其均数分别为81.11±17.25及31.06±4.43,P<0.05。侵袭性试验结果显示:Cyclin E阳性表达的原代乳腺癌细胞经24小时培养以后浸润到Transwell聚碳酸盐微孔滤膜下的细胞数的均值为:76.32±11.40/高倍视野;而Cyclin E阴性表达的原代乳腺癌细胞的均值为21.56±13.22/高倍视野;P<0.05。结论:Cyclin E的表达差异与原代乳腺癌细胞的侵袭性有关,Cyclin E高表达者其侵袭性显著强于Cyclin E低表达者。

Objective：To study the Correlation between expression of Cyclin E in breast cancer and invasion of primary breast cancer cells.Methods：1cm3 of the breast cancer tissue was taken got from operation,prepareing breast cancer cells suspension of origin generation,and detecting the expression level of Cyclin E by immunofluorescent technique.These cancer cells of origin generation were detected for invasion capability.Results：The invasion test presented that the average number of cells of origin generation with overexpression of Cyclin E infiltrating Transwell PC micropore film was 76.32±11.40 per high power field,the average number of cells of origin generation with negative expressivion of Cyclin E was 21.56±13.22 per high power field P 0.05.Conclusion：The expression of Cyclin E is related with the invasion of origin generation cells in breast cancer,the invasion of overexpression of Cyclin E is more powerful than negative expression of Cyclin E in breast cancer.