定量检测NSE和S-100推断脑挫伤时间的动物实验

Quantitative analysis of NSE,S-100 after experimental brain contusion for detecting the survival time

观察实验性大鼠脑挫伤后NSE、S－100的时间性变化规律，为法医学推断脑挫伤形成时间提供新的手段。采用改进的Feeney氏落体打击致Wistar大鼠脑挫伤模型，进行免疫组织化学SP法染色，并利用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量，SAS统计软件分析，结果显示：NSE阳性神经元灰度与面积在伤后1h至2d呈下降趋势，至伤后12h阳性细胞灰度、面积降到最小值（P＜0．01）；S－100阳性细胞面积和灰度伤后呈上升趋势，至伤后4dS－100阳性细胞灰度、面积达到最大值，与对照组及其它实验组比较均有显著性差异（P＜0．01），伤后5d仍保持较高水平；NSE、S－100免疫组织化学染色阳性细胞的数量、灰度、面积改变有时间规律。推断2d内脑挫伤可用NSE作为主要指标，推断2～5d的脑挫伤则以S－100改变为主。

It is very difficult to estimate the survival time died from brain injury. The changes of NSE and S－100 proteins of neurons and glias might give some help for estimating the survival time after brain injury. This paper devotes to study the changes of NSE, S－100 protein of neurons and glia cells after experimental brain contusion by using immunohistochemistry SP method combined with image quantitative analysis. The grey degrees and areas of NSE－positive cells decreased from the 1st hour to the 2nd day after injury and reached to the mininum level at the 12th hour while S－100－positive cells increased significantly at the 3rd hour after brain contusion, reached to the maximum level on the 4th day and kept at the high level until the 5the day. The changes of NSE, S－100 were regular along with various survival time after brain contusion. NSE can be used as the main marker at the early stage (1 hour to 2 day) of brain contusion while S－100 was useful mainly from 2 to 5 day.